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Updated: Jun 26, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
[Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)]
T B Blokhina1, E A Neshkova, G A Iarovaia
1Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2, Moscow, 123995 Russia.
Abstract:
A procedure of isolation of human blood plasma prekallikrein, coagulation factor XII and active fragment beta-XIIa has been developed. This procedure includes the traditional chromatography steps and FPLC. Disc-electrophoresis revealed that the preparations of factor XII and beta-XIIa were homogeneous. Their specific activity was 70.6 U and 2.5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities.
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