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Published on: September 9, 2012
Influence of the sampling technique on the measurement of peritoneal fibrinolytic activity
J Neudecker1, T Junghans, S Ziemer
1Department of General-, Visceral-, Vascular- and Thoracic Surgery, Medical Faculty of the Humboldt-University in Berlin, Berlin, Germany.
Objective:
To establish the influence of the peritoneal sampling technique on the measurement of fibrinolytic capacity.
Design:
Clinical study.
Setting:
University hospital, Germany.
Subjects:
40 peritoneal biopsy specimens were taken from 10 patients who were having elective colorectal resections.
Interventions:
Peritoneal biopsy specimens were taken either with a biopsy punch (n = 20) or manually with forceps and scissors (n = 20).
Main Outcome Measures:
Extent of agreement in fibrinolytic activities between specimens taken with biopsy punch and manually. Major endpoint-peritoneal tissue plasminogen activator (t-PA) activity. Minor endpoints-peritoneal tissue plasminogen activator concentration, and concentration and activity of plasminogen activator inhibitior type 1 (PAT-1).
Results:
Intra-assay agreement and the extent of agreement between the groups were evaluated by the method of Bland and Altman. Correlation of repeated measurements of t-PA and PAI-1 concentrations and activities from the same sample using the same ELISA kit was high (r = 0.93-0.99, p < 0.01). t-PA activities and concentrations between the groups correlated poorly (r= 0.60 and 0.66, p < 0.01) while no correlation at all was seen for PAI-1 concentration and activity between the groups (r = 0.6 and 0.1, p = 0.2 and 0.9). The mean differences between the groups ranged from -27% to -4.8%.
Conclusion:
The sampling technique considerably affects the measurement of peritoneal fibrinolytic activity.
