Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear factor-kappaB in middle ear epithelial cells: a methodological study using an ELISA.

Therese Ovesen1, Thomas Q Barrett

  • 1ENT Department, Aarhus University Hospital, Aarhus, Denmark. toves@akh.aaa.dk

Acta Oto-Laryngologica
|April 19, 2003
PubMed
Summary

This study measured nuclear factor-kappaB (NF-kappaB) in middle ear cells using ELISA. Lipopolysaccharide (LPS) significantly increased NF-kappaB levels, suggesting its role in otitis media with effusion inflammation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Learning curve in autofluorescence-guided thyroid surgery.

Frontiers in endocrinology·2026
Same author

Differences in upper airway endotype among phenotypically different pediatric OSA patients.

Sleep medicine·2026
Same author

Three-dimensional scan versus conventional mould of outer ear in fabricating earmolds for hearing aids: a randomized controlled trial.

International journal of audiology·2026
Same author

Global airways: a Danish nationwide real-life registry of biologic therapy for chronic rhinosinusitis with nasal polyps.

Frontiers in allergy·2026
Same author

[Chronic obstructive sleep apnoea].

Ugeskrift for laeger·2025
Same author

Iatrogenic smell and taste loss after surgery and anaesthesia.

Danish medical journal·2025

Area of Science:

  • Biochemistry
  • Cell Biology
  • Otolaryngology

Background:

  • Otitis media with effusion (OME) involves complex intracellular mechanisms.
  • Nuclear factor-kappaB (NF-kappaB) is a key transcription factor in inflammatory pathways.

Purpose of the Study:

  • To detect and quantify NF-kappaB in middle ear epithelial cells.
  • To investigate the role of NF-kappaB in OME pathogenesis.

Main Methods:

  • Enzyme-Linked Immunosorbent Assay (ELISA) was employed to measure NF-kappaB.
  • Cultured rabbit middle ear epithelial cells were stimulated with lipopolysaccharide (LPS).
  • Western blot analysis was used for result confirmation.

Main Results:

  • NF-kappaB was quantifiable in a concentration range of 16-32 ng/microg total cell protein.

Related Experiment Videos

  • LPS exposure significantly enhanced NF-kappaB levels.
  • NF-kappaB concentration increased with LPS exposure duration.

    • Conclusions:

    • NF-kappaB is measurable in middle ear epithelial cells.
    • LPS significantly upregulates NF-kappaB, indicating its involvement in OME.
    • Further research is needed to elucidate NF-kappaB's precise role in middle ear inflammation.