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Updated: Sep 26, 2026
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
Isolation and characterisation of the phospholipase B gene of Cryptococcus neoformans var. gattii
G Nicolas Latouche1, Tania C Sorrell, Wieland Meyer
1Molecular Mycology Laboratory, CIDM, Westmead Hospital, ICPMR, Level 3 Room 3114A, Darcy Road, NSW 2145, Australia.
Abstract:
Cryptococcus neoformans var. gattii (serotypes B and C) is a human pathogen, ecologically, biochemically, clinically and genetically different from C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D). The phospholipase B (PLB1) gene from serotypes B and C was isolated and characterised. It resembled the serotype A and D genes, with an overall sequence homology of more than 85%. The respective open reading frames were 2236 bp (serotype B) and 2239 bp (serotype C) in length. Each contained six introns and encoded a 68-kDa protein destined for secretion. PLB1 was located on the second smallest chromosome in both serotypes. Gene expression, measured as mRNA, was not regulated by temperature, pH or exogenous nutrients.
