Related Experiment Video
Updated: Sep 26, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Genes from Debaryomyces hansenii increase salt tolerance in Saccharomyces cerevisiae W303
Catarina Prista1, Ana Soeiro, Paul Vesely
1Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisbon, Portugal.
Abstract:
The yeast Debaryomyces hansenii has been chosen as a model for molecular studies of tolerance to NaCl. A gene library was built and transformants of Saccharomyces cerevisiae W303 containing genes from D. hansenii were selected for their ability to grow in the presence of high concentrations of NaCl and/or low concentrations of KCl. In three of these transformants 500 mM NaCl improved growth at pH 7.6 like in D. hansenii but not in S. cerevisiae. One of the plasmids restored growth at 50 microM KCl and K(+) uptake in a mutant of S. cerevisiae lacking genes that encode K(+) transporters.
More Related Videos
10:08Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
07:38Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Related Concept Videos
Bioreactor Controls-III
Production of Alcohol
Microbes in Beverage Production