Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polycistronic gene expression in yeast versus cryptic promoter elements.

Katrin Hecht1, James E Bailey, Wolfgang Minas

  • 1Institute of Biotechnology, ETH-Zürich, Switzerland. hecht@biotech.biol.ethz.ch

FEMS Yeast Research
|April 19, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Medication Affordability, Accessibility, and Availability in Care Transitions (Med AAAction) Study: Design and methods for a pharmacy-led trial implemented in low-income populations.

Contemporary clinical trials·2026
Same author

Healthy eating and active living to reverse diabetes (HEAL Diabetes-USA): a randomized controlled pilot study protocol to assess the feasibility of a weight loss intervention for diabetes remission.

Contemporary clinical trials communications·2026
Same author

Comparative Effectiveness of Diabetes Self-Care Interventions in African-American Adults: A Three-Arm Randomized Controlled Trial.

Journal of general internal medicine·2025
Same author

Full subsidy and direct delivery of essential chronic disease medications for low-income populations: A pilot randomized controlled trial.

Journal of the National Medical Association·2025
Same author

Building a payment model for health coaching in primary care: lessons from Tennessee.

The American journal of managed care·2025
Same author

Industrializing Biocatalysis.

Chimia·2025

Polycistronic gene expression in Saccharomyces cerevisiae is challenging. Specific 5' untranslated regions (5'UTRs) can enhance reporter gene expression in yeast, acting as promoters regulated by carbon sources.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Yeast Genetics

Background:

  • Saccharomyces cerevisiae is a key host for biotechnology, but expressing complex heterologous pathways is difficult.
  • Polycistronic gene expression is a potential solution, but requires effective intergenic elements.
  • Short 5' untranslated regions (5'UTRs) have shown cap-independent translation in vitro.

Purpose of the Study:

  • To investigate the utility of 5'UTRs as linkers in polycistronic constructs for yeast.
  • To evaluate the expression levels of genes at different positions within polycistronic units.
  • To determine if specific 5'UTRs can drive promoterless gene expression and respond to carbon sources.

Main Methods:

  • Constructed polycistronic vectors in Saccharomyces cerevisiae with reporter genes (GFP) at various positions.

Related Experiment Videos

  • Utilized specific 5'UTRs (5'TFIID, 5'HAP4, 5'YAP1, 5'L-A) as intergenic linkers.
  • Assayed green fluorescence protein (GFP) expression in yeast grown on glucose, raffinose, and galactose.

    • Main Results:

    • Ribosomal read-through alone is insufficient for effective polycistronic expression in vivo.
    • 5'TFIID and 5'HAP4 significantly improved expression of a second gene in bicistronic constructs.
    • 5'TFIID, 5'HAP4, and 5'YAP1 drove expression of promoterless genes and showed carbon source-dependent induction (raffinose/galactose > glucose).

    Conclusions:

    • Specific 5'UTRs function as effective linkers and can drive gene expression in polycistronic yeast systems.
    • These 5'UTRs possess promoter-like activity, enabling carbon source-regulated expression.
    • This finding offers a novel tool for engineering complex metabolic pathways in Saccharomyces cerevisiae.