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Updated: Sep 26, 2026

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
[Enteric infections in Perugia's area: laboratory diagnosis, clinical aspects and epidemiology during 2001]
Daniele Crotti1, Maria Letizia D'Annibale, Giovanna Fonzo
1Sezione di Microbiologia e Parassitologia Clinica, A O di Perugia, P O R Silvestrini, Perugia, Italy.
Abstract:
During 2001 we analyzed 523 stool specimens (330 children, 193 adults) of patients with recent diarrhoea. We processed all specimens for protozoa, rotavirus, adenovirus, toxin A of C. difficile, and usual enteropathogen bacteria. Salmonella prevailed in 12.8% of cases (16.4% among children, 6.7% among adults), Campylobacter in 9.9% (11.5% and 7.3%), C. difficile toxin A producer in 11.3% (13.7% and 8.1%); other bacteria generally prevailed in 2.4%, protozoa in 2.7%. Among children rotavirus prevailed in 41.4%, adenovirus in 3.6%. Enteritis prevailed in children between 1 and 6 years of age. All pathogens were reported during all the seasons, but salmonellosis and campylobacteriosis were more frequent during spring and summer, rotavirus infections during the winter. Non particular and/or specific correlations could be observed between clinical manifestations and pathogen agents; anyway only bacteria were identified among invasive enterites. Vomitus was more frequent in rotavirus infections. Finally the Authors suggest a rational and efficacious methodology for diagnosis of presumptive infectious diarrhoeas.
Insights
This study analyzed 523 stool samples from patients with diarrhea, identifying common pathogens like rotavirus, Salmonella, and Campylobacter. Bacterial infections were more frequent in invasive enteritis cases.
Area of Science:
- Microbiology
- Infectious Diseases
- Pediatrics
Context:
- Analysis of 523 stool specimens from patients with recent diarrhea in 2001.
- Inclusion of both pediatric (330) and adult (193) patient populations.
- Comprehensive testing for a range of enteric pathogens including protozoa, rotavirus, adenovirus, Clostridium difficile toxin A, and common enteropathogenic bacteria.
Purpose:
- To identify the prevalence of various enteric pathogens in patients presenting with diarrhea.
- To investigate potential correlations between specific pathogens and clinical manifestations.
- To determine seasonal patterns of infectious diarrhea and suggest diagnostic methodologies.
Summary:
- Salmonella (12.8%), Campylobacter (9.9%), and Clostridium difficile toxin A (11.3%) were significant bacterial causes of diarrhea.
- Rotavirus (41.4%) was the predominant viral pathogen, particularly in children aged 1-6 years.
- While pathogens occurred year-round, bacterial infections peaked in spring/summer, and rotavirus in winter; invasive enteritis was exclusively bacterial.
Impact:
- Highlights the etiological agents responsible for infectious diarrhea in the studied population.
- Provides insights into seasonal variations of specific enteric infections.
- Suggests a diagnostic approach for managing infectious diarrheal diseases effectively.
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