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Updated: Sep 26, 2026
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Published on: August 12, 2019
[Effects of lysophosphatidylcholine on expression of monocyte chemoattractant protein-1 in glomerular endothelial
1Third Department of Internal Medicine, Wakayama Medical University, Wakayama, Japan.
Abstract:
Glomerular endothelial cells(GEC) produce monocyte chemoattractant protein-1(MCP-1), which is considered to be an important factor for the recruitment of macrophages into the glomeruli. Recent reports have suggested an association between oxidized low-density lipoprotein(ox-LDL) and progression of glomerular disease. In this study, the effects of lysophosphatidylcholine(LysoPC), a modified phospholipid produced during LDL oxidation, on MCP-1 mRNA expression in cultured bovine GEC were examined. GEC from the 8th through 10th passages were used. LysoPC substantially increased expression of MCP-1 mRNA when compared to the control. These findings led us to examine the mechanism of LysoPC-induced MCP-1 expression in GEC. LysoPC-induced MCP-1 mRNA expression in GEC was suppressed by genistein and staurosporine. It was suggested that both the tyrosinekinase(TK) and proteinkinase C(PKC) pathways were involved in LysoPC-induced MCP-1 expression in GEC. MCP-1 mRNA induction by LysoPC was also attenuated by Vitamin E. This effect may be related to the beneficial effects of Vitamin E on experimental glomerular disease models. In conclusion, LysoPC increased MCP-1 expression in GEC. This phenomenon is believed to be mediated by both the TK and PKC signaling pathways, in contrast with other vascular endothelial cells. Vitamin E also attenuated LysoPC-induced MCP-1 expression in GEC.
Insights
Lysophosphatidylcholine (LysoPC), a product of oxidized low-density lipoprotein, increases monocyte chemoattractant protein-1 (MCP-1) expression in glomerular endothelial cells. Vitamin E mitigates this effect, suggesting a therapeutic role in glomerular disease.
Area of Science:
- Nephrology
- Cell Biology
- Biochemistry
Context:
- Glomerular endothelial cells (GEC) produce monocyte chemoattractant protein-1 (MCP-1), a key mediator of macrophage recruitment in glomeruli.
- Oxidized low-density lipoprotein (ox-LDL) is implicated in glomerular disease progression.
- Lysophosphatidylcholine (LysoPC) is a modified phospholipid generated during LDL oxidation.
Purpose:
- To investigate the effect of LysoPC on MCP-1 mRNA expression in cultured bovine GEC.
- To elucidate the signaling pathways involved in LysoPC-induced MCP-1 expression in GEC.
Summary:
- LysoPC significantly increased MCP-1 mRNA expression in cultured GEC.
- The induction of MCP-1 mRNA by LysoPC was suppressed by genistein and staurosporine, indicating involvement of tyrosine kinase (TK) and protein kinase C (PKC) pathways.
- Vitamin E attenuated LysoPC-induced MCP-1 mRNA expression, suggesting a potential protective mechanism.
Impact:
- LysoPC promotes MCP-1 expression in GEC via TK and PKC signaling pathways.
- Vitamin E's inhibitory effect on LysoPC-induced MCP-1 expression may explain its benefits in experimental glomerular disease models.
- Findings contribute to understanding the molecular mechanisms underlying glomerular inflammation and disease progression.
