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Updated: Sep 26, 2026
Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord
Published on: February 26, 2019
Scanning microscopic evaluation on the development of the cerebral cortex in embryonic mouse subjected to
X Z Sun1, M Inouye, S Hayasaka
1Department of Teratology and Genetics, Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
Abstract:
Morphological events occurring in the developing cerebral hemispheres of mice exposed to a single dose of 60Co gamma-irradiation 1.5 Gy on embryonic day 13 (E13) were evaluated by scanning microscope. Twenty-four hr after the exposure, both cell debris and surviving cells had poured out into the ventricular lumen. Radial glial fibers were more crumpled than in the controls. By day E15, proliferating cells in different stages of the cell cycle appeared in the ventricular zone. The glial fibers formed a network through the brain mantle. By E17 many migrating cells attached to the disorderly glial fibers appeared in the different layers of the thin cerebral mantle. These findings suggest that development of the glial fibers was interrupted as early as 24 hr after the single exposure, meaning that irradiation on the developing brain may disrupt neuronal migration.
