Electro-optic response of second-harmonic generation membrane potential sensors
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Updated: Aug 17, 2026

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
1Neurophysiologie et Nouvelles Microscopies, Ecole Superieure de Physique et de Chimie Industrielles, Institut National de la Santé et de la Recherche Scientifique, EPI 00-02, Paris, France.
Abstract:
We quantify and ascertain the nature of the second-harmonic generation (SHG) response of amphiphilic push-pull chromophores to a transmembrane electric field. Our technique is based on the application of an alternating field across labeled giant unilamelar vesicles. For chromophore responses that are purely electro-optic, our technique provides an estimate of photoinduced charge shifts based on the observed dispersion of the field response, in accord with a two-level perturbation theory. These results are applicable to the optimization of membrane potential sensors for SHG microscopy.
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