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Related Experiment Videos

Determination of zearalenone in corn: collaborative study.

O L Shotwell, M L Goulden, G A Bennett

    Journal - Association of Official Analytical Chemists
    |May 1, 1976
    PubMed
    Summary

    The Eppley method accurately detects zearalenone in corn, with improved precision at higher concentrations. This mycotoxin analysis is crucial for food safety.

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    Area of Science:

    • Agricultural Chemistry
    • Food Science
    • Analytical Chemistry

    Background:

    • Zearalenone is a mycotoxin produced by Fusarium fungi, posing a risk to animal and human health.
    • Accurate quantification of zearalenone in agricultural commodities is essential for regulatory compliance and food safety.

    Purpose of the Study:

    • To evaluate the performance of the Eppley method for determining zearalenone in corn.
    • To assess the method's accuracy, precision, and reliability across various contamination levels.

    Main Methods:

    • Collaborative study involving 22 laboratories analyzing corn samples spiked with zearalenone (100-2000 mug/kg).
    • Statistical analysis of results from 16 participating laboratories using the Eppley method.
    • Analysis of naturally contaminated corn samples to assess real-world applicability.

    Main Results:

    • The Eppley method demonstrated detection of zearalenone at 300 mug/kg by most collaborators.
    • Average recoveries ranged from 88% to 129%, with better precision at higher concentrations (CV 27.0% at 2000 mug/kg).
    • Naturally contaminated samples showed mean zearalenone levels from 431 to 7622 mug/kg.

    Conclusions:

    • The Eppley method is a reliable technique for zearalenone analysis in corn.
    • The method's performance is acceptable, particularly at higher contamination levels.
    • Densitometric measurement on TLC plates shows potential for zearalenone quantification.

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