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Related Experiment Videos

Same serial section correlative light and energy-filtered transmission electron microscopy.

Ying Ren1, Michael J Kruhlak, David P Bazett-Jones

  • 1Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|April 22, 2003
PubMed
Summary

This study presents a successful technique for correlative light and electron microscopy, enabling precise cell identification and serial sectioning for detailed 3D suborganelle analysis.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Electron Microscopy

Background:

  • Correlative imaging using light and electron microscopy is challenging.
  • Requires significant patience and technical expertise.
  • Identifying specific cells for ultrastructural analysis is difficult.

Purpose of the Study:

  • To describe a high-success-rate technique for correlative light and electron microscopy.
  • To enable precise identification and serial sectioning of single cells.
  • To facilitate 3D suborganelle analysis using transmission electron microscopy.

Main Methods:

  • Light microscopy for initial cell identification.
  • Embedding and thin-sectioning of identified cells.
  • Serial section collection for transmission electron microscopy (TEM).

Related Experiment Videos

  • Imaging via conventional or energy-filtered TEM.

    • Main Results:

    • A reliable method for correlative light and electron microscopy was established.
    • High-quality, uninterrupted serial sections were obtained.
    • The technique allows for detailed 3D reconstruction at the suborganelle level.

    Conclusions:

    • The described technique significantly simplifies correlative imaging.
    • It enhances the ability to study cellular ultrastructure in 3D.
    • This method is valuable for detailed cell biology research.