High-performance liquid chromatographic determination of kanamycin

Area of Science:

• Analytical Chemistry
• Chromatography
• Biochemistry

Background:

• Kanamycin is an aminoglycoside antibiotic used to treat bacterial infections.
• Accurate quantification of kanamycin A and B is crucial for therapeutic drug monitoring and quality control.
• Existing methods may lack the speed, selectivity, or sensitivity required for routine analysis.

Purpose of the Study:

• To develop and validate a rapid, selective, and precise liquid chromatographic method for the simultaneous determination of kanamycin A and B.
• To establish sensitive detection limits for both kanamycin A and B.
• To assess the reproducibility and precision of the developed analytical method.

Main Methods:

• Utilized a pellicular cation exchanger for chromatographic separation of kanamycin A and B.
• Employed post-column on-line derivatization for fluorescence monitoring.
• Optimized chromatographic conditions for efficient separation and detection.

Main Results:

• Achieved simultaneous and independent determination of kanamycin A and B.
• Established a detection limit of <0.35 µg for kanamycin B in the presence of 7 µg kanamycin A.
• Demonstrated a detection limit of <20 ng for kanamycin A.
• Reported system reproducibility of ~1% (2θ) and sample preparation precision of ~6% (2θ).
• Completed chromatographic analysis in under 15 minutes per sample.

Conclusions:

• The developed liquid chromatographic method is fast, selective, and precise for simultaneous kanamycin A and B analysis.
• The method offers high sensitivity, making it suitable for detecting low concentrations of kanamycin.
• This technique provides reliable and reproducible results for both standard and process samples.