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Interfacing the orbitrap mass analyzer to an electrospray ion source.

Mark Hardman¹, Alexander A Makarov

¹Thermo Masslab Ltd., Crewe Road, Wythenshawe, Manchester, M23 9BE, UK.

Analytical Chemistry

|April 23, 2003

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The Orbitrap mass analyzer, using a novel electrostatic field, achieves high resolution and mass accuracy. It can be coupled with electrospray ionization for sensitive analysis.

Area of Science:

Analytical Chemistry
Physical Chemistry
Instrumental Analysis

Background:

The Orbitrap mass analyzer utilizes an electrostatic field for ion trapping and mass analysis.
Traditional Orbitrap designs have limitations in ion injection and source coupling.

Purpose of the Study:

To demonstrate the coupling of an Orbitrap mass analyzer with a continuous electrospray ionization source.
To characterize the performance of this integrated system.

Main Methods:

Development of an RF-only quadrupole for external ion accumulation and injection.
Utilizing fast Fourier transform (FFT) of image currents for mass-to-charge ratio determination.
Coupling the Orbitrap trap with an electrospray ion source.

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Main Results:

Achieved high mass resolving power up to 150,000 FWHM.
Demonstrated mass accuracies within a few parts per million.
Enabled an 8-fold relative mass range with good sensitivity.

Conclusions:

The coupled Orbitrap system is suitable for sensitive analysis.
The developed ion injection method allows for efficient coupling with continuous ion sources.
Space-charge capacity of the accumulation quadrupole limits the maximum ion number for analysis.