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Increased basigin in bleomycin-induced lung injury
Tomoko Betsuyaku1, Kenji Kadomatsu, Gail L Griffin
1Department of Medicine and Cell Biology, Washington University School of Medicine, Barnes-Jewish Hospital, St. Louis, Missouri, USA. bytomoko@med.hokudai.ac.jp
American Journal of Respiratory Cell and Molecular Biology
|April 23, 2003
Summary
Basigin, a protein found in lung tissue, increases with bleomycin-induced lung injury. This suggests basigin may play a role in fibrotic lung diseases like diffuse alveolar injury.
Area of Science:
- Pulmonary Medicine
- Cell Biology
- Biochemistry
Background:
- Basigin is present in various tissues and implicated in wound healing and tumor progression.
- Its role in fibro-inflammatory lung conditions is not well understood.
Purpose of the Study:
- To investigate basigin expression in bleomycin-induced lung injury, a model of diffuse alveolar damage and fibrosis.
- To determine if basigin is upregulated in fibrotic lung processes.
Main Methods:
- Induction of lung injury using intratracheal bleomycin in a mouse model.
- Analysis of basigin expression via Western blot, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and in situ hybridization.
- Examination of basigin in lung tissue, bronchoalveolar lavage fluid (BALF), and RIPA-insoluble fractions.
Main Results:
- Basigin expression was significantly increased in fibrotic areas, alveolar macrophages, and bronchiolar epithelium 14 days post-bleomycin.
- Increased basigin levels were detected in RIPA-insoluble fractions of bleomycin-treated lungs.
- Basigin mRNA levels were elevated, and basigin was found in various forms in BALF after bleomycin treatment.
Conclusions:
- Bleomycin-induced lung injury is associated with elevated basigin expression in lung tissue and BALF.
- Basigin deposition occurs at fibrotic sites.
- These findings suggest a potential role for basigin in the pathogenesis of diffuse alveolar injury and lung fibrosis.