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Bone marrow stromal cells and their use in regenerating bone.
Ranieri Cancedda1, Maddalena Mastrogiacomo, Giordano Bianchi
1Istituto Nazionale per la Ricerca sul Cancro, Centro Biotecnologie Avanzate, Università di Genova, Largo R. Benzi 10, 16132 Genova, Italy.
Novartis Foundation Symposium
|April 24, 2003
Summary
Tissue engineering using bone marrow stromal cells (BMSCs) and bioceramics shows promise for repairing large bone defects. This approach facilitates new bone formation and vascularization, offering a significant improvement for critical-sized bone injuries.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Orthopedic Surgery
Background:
- Large bone defects pose significant clinical challenges.
- Tissue engineering offers a promising solution by combining cells and scaffolds.
- Bone marrow stromal cells (BMSCs) are a viable cell source for bone regeneration.
Purpose of the Study:
- To evaluate the efficacy of autologous BMSC/bioceramic composites in repairing critical-sized long bone defects.
- To investigate the bone healing process in vivo using sheep and human models.
Main Methods:
- Autologous BMSC/bioceramic composites were implanted in sheep tibial defects.
- Healing was assessed by observing bone formation and implant integration.
- Similar composites were used to treat three human patients with long bone defects, followed by external fixation.
Main Results:
- In sheep, bone formation occurred on the implant surface, within the canal, and within the bioceramic pores.
- Implant fissures facilitated bone ingrowth.
- Human patient outcomes were promising after over three years of follow-up.
Conclusions:
- Culture-expanded osteoprogenitor cells combined with hydroxyapatite bioceramics represent a significant advancement in repairing critical-sized long bone defects.
- This tissue engineering strategy promotes vascularized bone regeneration.