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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayer
Kenichi Ataka1, Joachim Heberle
1Forschungszentrum Jülich, IBI-2: Structural Biology, 52425 Jülich, Germany.
Abstract:
Functional infrared difference spectra of a protein monolayer are recorded by exploiting the surface enhancement exerted by a modified gold surface. The noble metal is used as the working electrode to induce redox-linked structural changes in the single layer of cytochrome c. The observed differences in the relative band intensities as compared to the difference spectrum of cytochrome c in solution are attributed to orientational effects of the adsorbed protein.
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