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Related Experiment Videos

Oocyte-induced haploidization.

Gianpiero D Palermo1, Takumi Takeuchi, Zev Rosenwaks

  • 1Takumi Takeuchi, Zev Rosenwaks The Centre for Reproductive Medicine and Infertility, Weill Medical College of Cornell University, New York, NY 10021, USA. gdpalerm@med.cornell.edu

Reproductive Biomedicine Online
|April 24, 2003
PubMed
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Nuclear transplantation successfully created viable oocytes from aged ones in mice and humans. This technique offers a potential solution for age-related infertility by restoring normal oocyte function and ploidy.

Area of Science:

  • Reproductive biology
  • Cellular and molecular biology
  • Genetics

Background:

  • Age-related oocyte aneuploidy is a significant cause of infertility.
  • Current solutions like oocyte/embryo selection have limitations.
  • Nuclear transplantation offers an alternative approach to generating viable gametes.

Purpose of the Study:

  • To describe the technical approach for treating age-related oocyte aneuploidy using nuclear transplantation.
  • To evaluate the efficiency and outcomes of nuclear transplantation in mouse and human oocytes.
  • To assess the potential of this technique for producing normal oocytes in cases of infertility.

Main Methods:

  • Germinal vesicle (GV)-stage oocytes and somatic cells were collected from mice and humans.

Related Experiment Videos

  • Somatic nuclei were transferred into enucleated oocytes via micromanipulation.
  • Reconstituted oocytes were electrofused, cultured for maturation, and assessed for fertilization and ploidy.
  • Nuclear transplantation involved using GV ooplasts and either GV or somatic nuclei.

    • Main Results:

    • Nuclear transplantation achieved high efficiency in both mouse (approx. 80%) and human (approx. 70%) oocytes.
    • Reconstituted oocytes showed comparable maturation rates and aneuploidy incidence to controls.
    • Reconstituted human oocytes were successfully fertilized by intracytoplasmic sperm injection (ICSI) in 52% of cases.
    • Polar body extrusion occurred in over 40% of oocytes receiving nuclear transplants.
    • The technique did not increase chromosomal anomalies; reconstituted oocytes maintained normal ploidy.

    Conclusions:

    • Nuclear transplantation is a viable technique for generating normal oocytes, even from aged or aneuploid ones.
    • This procedure may offer a realistic solution for age-related infertility.
    • While similar to cloning, it results in a unique individual with contributions from both parental genomes.