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Personal pathways to embryonic stem cells.

R G Edwards1

  • 1Editor, Reproductive BioMedicine Online Online, Duck End Farm, Dry Drayton, Cambridge CB3 8DB, UK.

Reproductive Biomedicine Online
|April 24, 2003
PubMed
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Early research established immortal rabbit embryo stem cell lines with potential for differentiation and chimaera formation. These stem cells demonstrated multipotency and enabled transgenesis, paving the way for future stem cell applications.

Area of Science:

  • Developmental Biology
  • Stem Cell Research
  • Cell Biology

Background:

  • Research into embryo stem cells in vitro commenced in 1963 using rabbit embryos.
  • Early work focused on disaggregated blastomeres and whole-embryo cultures.

Purpose of the Study:

  • To derive and characterize long-lasting, stable embryo stem cell lines.
  • To assess the embryological potency and differentiation capacity of these stem cells.
  • To explore the potential of embryo stem cells in therapeutic applications.

Main Methods:

  • Culturing disaggregated blastomeres and inner cell masses from rabbit embryos.
  • Injecting inner cell masses into mouse blastocysts to assess chimaera formation.
  • Growing mouse and rat stem cells from blastocysts and post-implantation embryos.

Related Experiment Videos

  • Evaluating stem cell colonization of bone marrow in irradiated mice and mice with anemia.

    • Main Results:

    • Established immortal, stable embryo stem cell lines from rabbit inner cell mass and embryonic disc.
    • Demonstrated differentiation into various cell types and successful chimaera formation in mice.
    • Showed successful bone marrow and spleen colonization by mouse and rat stem cells in vivo.
    • Observed weak colonization in non-irradiated recipients without signs of inflammation or cancer.

    Conclusions:

    • Rabbit embryo stem cells possess multipotency and can contribute to various tissues.
    • Embryo stem cell technology has implications for cell lineage studies and transgenesis.
    • Stem cells derived from early embryos show potential for bone marrow reconstitution and therapeutic applications.