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Related Experiment Video

Updated: Dec 29, 2025

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Metaphase II transgenesis.

Anthony C F Perry¹

¹Advanced Cell Technology, Worcester, Massachusetts 01605, USA. tony@cdb.riken.go.jp

Reproductive Biomedicine Online

|April 24, 2003

Summary

This summary is machine-generated.

A new method, mII transgenesis, efficiently introduces transgenes into mammals. This technique, using sperm heads and DNA injected into oocytes, matches established methods and aids in creating disease models.

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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

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Published on: March 31, 2015

Area of Science:

Mammalian genetics
Molecular biology
Reproductive biology

Background:

Over 11 methods exist for introducing heritable exogenous DNA (transgenes) into mammals since 1974.
Existing methods have varying efficiencies and applications in mammalian genome engineering.

Purpose of the Study:

To review existing transgene delivery methods.
To introduce and evaluate a novel method: mII transgenesis.
To highlight the potential of mII transgenesis for mammalian genome engineering.

Main Methods:

Review of established mammalian transgenesis techniques.
Demonstration of mII transgenesis via microinjection of membrane-depleted sperm heads and transgene DNA into metaphase II oocytes.

Main Results:

mII transgenesis demonstrates efficiency comparable to traditional pronuclear microinjection.
The method involves direct injection into metaphase II oocytes using modified sperm.
Successfully introduces potentially heritable exogenous DNA into mammals.

Conclusions:

mII transgenesis is a viable and efficient new method for creating transgenic mammals.
This technique facilitates the delivery of large transgenes, crucial for generating disease models.
Offers a promising alternative for advancing mammalian genome engineering paradigms.