Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quick and reversible inhibition of soybean root nodule growth by nitrate involves a decrease in sucrose supply to

Hiroyuki Fujikake1, Akihiko Yamazaki, Norikuni Ohtake

  • 1Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan.

Journal of Experimental Botany
|April 24, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of 12-Week Sorghum Consumption on Visceral Fat Area and Metabolic Parameters in Japanese Adults: An Exploratory Single-Arm Trial.

Nutrients·2026
Same author

Correction: Current practice and awareness of perioperative do-not-attempt-resuscitation orders: a single-center retrospective survey and complete questionnaire survey.

Journal of anesthesia·2025
Same author

Genetic control of seed iron and zinc concentration in Rwandan common bean population revealed by the Genome Wide Association Study (GWAS).

Breeding science·2025
Same author

Inhibiting <i>Escherichia coli</i> Growth by Optimized Low-Power Microwave Irradiation-Delivery of Ag and Au Nanoparticles.

Molecules (Basel, Switzerland)·2025
Same author

Nutritional and functional profiles of aquaponic leaf lettuce across seasons.

Bioscience, biotechnology, and biochemistry·2025
Same author

Effects of Source on the Nitrogen Uptake, Allocation Patterns, and Performance of Strawberry (<i>Fragaria × ananassa</i> Duch.): A <sup>15</sup>N-Tracer Study.

Plants (Basel, Switzerland)·2025

Nitrate supply reversibly inhibits soybean nodule growth by reducing photosynthate allocation to nodules. This effect is linked to decreased carbon flow, impacting nodule cell expansion and overall development.

Area of Science:

  • Plant Biology
  • Agronomy
  • Biochemistry

Background:

  • Soybean nodule development is crucial for nitrogen fixation.
  • Nitrate availability is known to influence nodule formation and function.
  • Understanding nitrate's impact on nodule growth is key for optimizing crop yields.

Purpose of the Study:

  • To investigate the effect of nitrate on soybean nodule growth dynamics.
  • To determine the role of photosynthate translocation in nitrate-induced nodule growth inhibition.
  • To elucidate the reversibility and mechanisms of nitrate's impact on nodule development.

Main Methods:

  • Hydroponic culture of soybean roots with computer microscopy for nodule diameter measurement.
  • Controlled environmental conditions and nutrient manipulation (nitrate supply/withdrawal).

Related Experiment Videos

  • Use of (11)C or (14)C-labelled carbon dioxide to trace photosynthate distribution.

    • Main Results:

    • Nitrate supply (5 mM) rapidly and reversibly inhibited nodule growth by halting cell expansion.
    • Nodule growth resumed normal rates upon nitrate withdrawal.
    • Nitrate supply decreased photosynthate partitioning to nodules from 9% to 4%, despite stimulating root translocation.

    Conclusions:

    • A decrease in photoassimilate supply to nodules is implicated in the rapid and reversible inhibition of soybean nodule growth by nitrate.
    • Nitrate's inhibitory effect is partially mitigated by exogenous sucrose, suggesting a role for carbon availability.
    • The findings highlight the complex interplay between nitrogen nutrition and carbon allocation in symbiotic plant development.