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Related Experiment Videos

Graft-vs-host disease after solid organ transplant.

H Evin Gulbahce1, Charlotte A Brown, Myra Wick

  • 1Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Fairview-University Medical Center, Minneapolis, USA.

American Journal of Clinical Pathology
|April 25, 2003
PubMed
Summary

Graft-versus-host disease (GVHD) diagnosis in solid organ transplant recipients can be challenging. Molecular testing for donor chimerism aids in confirming GVHD, especially in ambiguous histological cases.

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Area of Science:

  • Transplantation immunology
  • Gastroenterology
  • Histopathology

Background:

  • Graft-versus-host disease (GVHD) is a potential complication following solid organ transplantation.
  • Histological diagnosis of GVHD can be challenging due to overlapping features with other conditions.

Purpose of the Study:

  • To evaluate the diagnostic utility of histological findings and molecular testing for GVHD in solid organ transplant recipients.
  • To assess the timing of GVHD diagnosis relative to transplantation and blood product transfusion.

Main Methods:

  • Retrospective review of 10 solid organ transplant recipients with a histological diagnosis of GVHD.
  • Analysis of transplant details, HLA matching, blood product transfusion history, and biopsy results (gastrointestinal, skin, liver).

Related Experiment Videos

  • Molecular testing for donor chimerism using the DIS80 marker in one case.

    • Main Results:

    • All patients showed grade I to IV acute GVHD in biopsy specimens.
    • Six patients were diagnosed within 3 months of transfusion/transplantation; four had a distant history.
    • Donor lymphocytes (chimerism) were detected in colonic tissue and blood in one patient.

    Conclusions:

    • Histological findings of GVHD are suggestive but not pathognomonic.
    • Molecular demonstration of donor lymphocytes (chimerism) is valuable for confirming GVHD in equivocal cases.
    • GVHD can occur within the expected timeframe or with a delayed presentation after solid organ transplantation.