Turkey microsatellite loci from an embryonic cDNA library

¹Animal Biotechnology Center, University of Minnesota, St. Paul, Minnesota 55108, USA.

|April 25, 2003

Summary

Researchers developed new polymorphic microsatellite markers from turkey expressed sequence tags (EST). These markers are valuable for genetic mapping and understanding evolutionary relationships between turkey and chicken species.

Area of Science:

Genomics
Comparative genomics
Molecular genetics

Background:

Expressed sequence tags (ESTs) with microsatellites are crucial for genetic and syntenic mapping.
They facilitate comparative mapping studies across species.
Microsatellite markers are widely used in genetic research.

Purpose of the Study:

To develop novel polymorphic microsatellite markers from turkey expressed sequence tags (ESTs).
To identify ESTs with homology to known coding regions for comparative mapping.
To establish a resource population for genetic linkage mapping in turkeys.

Main Methods:

Construction and screening of a turkey embryonic cDNA library using microsatellite probes ((CA/TG)15, (GA/CT)15, (AGG)10, (AAAC)7).
Sequencing of 128 cDNA clones to identify microsatellite-containing loci.
BLAST nucleotide analysis to determine homology with known sequences.
Testing polymorphism of new loci on commercial turkey lines.

Main Results:

Identified 42 new loci containing microsatellites from 128 sequenced turkey ESTs.
Fifteen turkey ESTs showed significant homology to mammalian or avian coding regions, with five matching chicken transcripts.
Twenty-seven ESTs represented novel sequences.
Thirty-one of the 42 new loci were polymorphic in commercial turkey lines.

Conclusions:

Developed 42 novel microsatellite markers from turkey ESTs, with 31 showing polymorphism.
These markers are valuable for genetic linkage mapping in turkeys.
Comparative mapping of these markers will elucidate evolutionary divergence between turkey, chicken, and other species.

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Related Experiment Videos

Turkey microsatellite loci from an embryonic cDNA library

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

Related Concept Videos

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