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Related Experiment Videos

NusA modulates intragenic termination by different pathways.

M Stella Carlomagno1, Annagiulia Nappo

  • 1Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy. carloma@unina.it

Gene
|April 25, 2003
PubMed
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Bacterial transcription termination relies on NusA, which aids in stopping RNA synthesis at intragenic sites. NusA works with other factors like NusB and ribosomes to regulate termination efficiency and prevent premature transcript release.

Area of Science:

  • Bacterial transcription-translation coupling
  • Gene regulation
  • RNA polymerase function

Background:

  • Intragenic terminators are activated when translation and transcription are uncoupled in bacteria.
  • NusA, NusB, and NusE/S10 are key factors in bacterial transcription termination.
  • The hisG cistron contains two tandem terminators, GTTE1 (suboptimal) and GTTE2 (canonical Rho-dependent).

Purpose of the Study:

  • To investigate the role of NusA in intragenic transcription termination.
  • To analyze how NusA functions with other factors (NusB, NusE/S10, ribosomes) at different terminators.
  • To understand how translation influences termination at the GTTE1 terminator.

Main Methods:

  • Analysis of intragenic termination using two tandem terminators (GTTE1, GTTE2) in the hisG cistron.

Related Experiment Videos

  • In vitro and in vivo experiments to assess termination efficiency.
  • Investigating the influence of translation, ribosomal components, and Nus factors (NusA, NusB, NusE/S10).

    • Main Results:

    • NusA is essential for efficient termination at both GTTE1 and GTTE2.
    • At GTTE1, NusA's function depends on ribosome presence and the position of the stop codon relative to boxA.
    • NusA-stimulated pausing is sufficient for termination at GTTE2, and NusA prevents premature termination of translated transcripts.

    Conclusions:

    • NusA plays a critical role in regulating intragenic transcription termination in bacteria.
    • NusA interacts with nascent mRNA, other Nus factors, and ribosomes to modulate termination.
    • NusA may program RNA polymerase to terminate transcription at specific mRNA sites.