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Related Experiment Videos

Profiling local gene expression changes associated with Eimeria maxima and Eimeria acervulina using cDNA microarray.

W Min1, H S Lillehoj, S Kim

  • 1Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Service, U.S. Department of Agriculture, Building 1040, BARC-East, Beltsville, MD 20705, USA.

Applied Microbiology and Biotechnology
|April 25, 2003
PubMed
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Eimeria parasites trigger distinct immune responses in chickens. Eimeria acervulina and Eimeria maxima infections alter gene expression in intestinal lymphocytes, revealing insights into host-parasite interactions and immunity.

Area of Science:

  • Avian immunology
  • Parasitology
  • Molecular biology

Background:

  • Eimeria parasites are significant avian pathogens, causing coccidiosis.
  • Eimeria species exhibit specific intestinal invasion sites and elicit tailored host immune responses.
  • Understanding these host-parasite interactions is crucial for developing effective immunity.

Purpose of the Study:

  • To investigate global transcriptional changes in chicken intestinal intraepithelial lymphocytes (IELs) following infection with Eimeria acervulina or Eimeria maxima.
  • To identify specific genes and cytokine mRNAs involved in the host immune response to Eimeria infection.
  • To compare the immune responses induced by primary versus secondary infections and by different Eimeria species.

Main Methods:

  • Oral inoculation of chickens with Eimeria acervulina or Eimeria maxima.

Related Experiment Videos

  • Monitoring global gene expression changes in intestinal IELs using cDNA microarrays with 400 unique chicken genes.
  • Analyzing differential gene transcript regulation (up- or down-regulation) after primary and secondary infections.

    • Main Results:

    • Significant up- or down-regulation of multiple gene transcripts was observed following Eimeria infections.
    • Primary infections generally induced more gene expression changes than secondary infections.
    • Eimeria acervulina infection resulted in more transcriptional changes compared to Eimeria maxima infection.
    • Similar changes in cytokine mRNA levels were noted for both species after primary infection.
    • A common set of transcripts was identified with enhanced or repressed expression in IELs for both parasite species.

    Conclusions:

    • Microarray analysis is a powerful tool for studying host-parasite interactions in Eimeria-infected chickens.
    • The study identified key gene expression patterns associated with the host immune response to Eimeria.
    • Findings contribute to a better understanding of protective immunity against avian coccidiosis.