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Updated: Aug 17, 2026

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
Published on: March 27, 2011
[Nitrate assimilation activity of yellow lupine root nodules ?]
S F Izmaĭlov1, R K Bruskova, S A Chechetka
1Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya ul. 35, Moscow, 127176 Russia.
Abstract:
Low nitrate assimilation activity of the root nodules was demonstrated by assaying the nitrate reductase, glutamate synthase, glutamate dehydrogenase, and asparagine synthase activities, as well as the kinetics of 14C-labeled saccharose incorporation in the amino acids and amides of the cortex and the bacteroid-containing root nodule zones. Irrespective of the exogenous nitrogen concentration (0, 11.2, or 25 mM NO3-), nitrate concentration in the nodules was low as compared to the plant roots, leaves, and stems. This allowed us to propose the presence of structural and/or metabolic barriers in the nodules limiting nitrate accessibility and assimilation.
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