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Updated: Aug 14, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

[Preparation and identification of anti-fumonisin B1 monoclonal antibody]

Y Guo1, X Liu, J Liu

  • 1Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|April 26, 2003
PubMed

Spleen cells from Balb/c mice immunized with an artificially KLH-glutaraldehyde fumonisin B1(FB1) conjugate were fused with murine Sp2/0 myeloma cells. One hybridoma cell line secreting monoclonal antibody against FB1 was established after the fusion cells subcloned for 4 cycles. This antibody was designated as FB(1)4G4. The titer of the antibody from ascites was 1:1 x 10(7).

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...