Related Experiment Videos
[Selenoproteins in rats with chronic selenium intoxication]
1Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Wei Sheng Yan Jiu = Journal of Hygiene Research
|April 26, 2003
Summary
High selenium intake in rats reduced body weight gain and altered Se-containing enzyme activities, suggesting these changes indicate early selenium toxicity.
Area of Science:
- Biochemistry
- Toxicology
- Nutritional Science
Background:
- Selenium (Se) is an essential trace element crucial for various physiological functions.
- Excessive selenium intake can lead to toxicity, necessitating the identification of reliable biomarkers.
Purpose of the Study:
- To investigate the effects of adequate versus high dietary selenium on Wistar rats.
- To identify potential early biochemical markers for chronic selenium intoxication.
Main Methods:
- Male Wistar rats were fed diets with adequate (0.2 mg/kg) or high (0.5 mg/kg) selenium for 20 weeks.
- Tissue samples were analyzed for selenium concentration, Se-containing enzyme activities (eGPX, cGPX, ID I, PHGPX), and mRNA levels of selenoprotein P and W.
- Liver pathology was assessed.
Main Results:
- High selenium intake significantly reduced body weight gain and increased tissue selenium accumulation.
- Activities of key Se-containing enzymes (eGPX, cGPX, ID I, PHGPX) were significantly lower in the high Se group.
- No significant pathological changes were observed in the liver.
Conclusions:
- Reduced activities of specific Se-containing enzymes may serve as early biochemical indicators of chronic selenium intoxication.
- Dietary selenium levels significantly impact enzyme activity and physiological parameters in rats.