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Updated: Sep 26, 2026

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology
Published on: October 3, 2016
[Test for the performance of BL3 safety laboratory in prevention the diffusion of aerosol]
1Institute of Microbiology and Epidemicology, Academy of Military Medical Science, Beijing, 100071, China.
Abstract:
The performance of BL3 safety laboratory in preventing experimental aerosol diffusion and sterilizing microbe aerosols through high efficiency filter in a ventilation system was tasted. Aerosols of uranin, vibrio phage and Bacillus subtilis var. niger spores were used for the test. The results showed that the safety cabinets and the negative pressure room in the BL3 safety laboratory were effective. The sterilization efficiency by the combination of ozone, ultraviolet and high efficiency filter in a ventilation system of the laboratory was satisfactory. However, the position of fan and the leakage of ventilation ducts must be improved.

