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Updated: Aug 13, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
[Mechanism of injury by active oxygen radical to hemoglobin]
1Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Abstract:
The injury effects of O2-. and H2O2 on the hemoglobin were investigated. The results showed that the metal prosthetic group was injured by O2-. and H2O2, but the mechanism of O2-. and H2O2 effects was different. Through the charge neutralization O2-. destroying the static electrical interactions between prosthethic groups and amino acid residues, it thereby caused prosthetic group dropped. H2O2 had Fenton reaction with Fe2+ in prosthetic group, hence produced .OH. It can destroy the porphyrin structure at fixed point in short distance, so the prosthetic group was destroyed and non-reversible.
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