[Determination of sexual hormones in cosmetics by using Re-HPLC method]

A Re-HPLC method for the determination of seven kinds of sexual hormones, such as estriol in cosmetics was established. Zorbax C18 and Methanol-water were used as solid phase and liquid phase respectively. Methanol was used to extract the hormones in samples using a ultrasonic method. In the background with cosmetic samples, the relative standard deviations of those seven kinds of added sexual hormones were 1.2%-4.4% at low concentration and 2.1%-5.8% at high concentration. The recoveries rates of those hormones were 87.7%-103.1% and 86.6%-104.4%, respectively.