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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru
Miguel Talledo1, Irma N G Rivera, Erin K Lipp
1Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E Pratt St, Baltimore, Maryland 21202, USA.
Abstract:
A Vibrio cholerae bacteriophage, family Myoviridae, was isolated from seawater collected from the coastal water of Lima, Peru. Genome size was estimated to be 29 kbp. The temperate phage was specific to V. cholerae and infected 12/13 V. cholerae O1 strains and half of the four non-O1/non-O139 strains tested in this study. Vibrio cholerae O139 strains were resistant to infection and highest infection rates were obtained in low nutrient media amended with NaCl or prepared using seawater as diluent.
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