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High-stability non-invasive autoclavable naked optical CO2 sensor.
Xudong Ge1, Yordan Kostov, Govind Rao
1Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
Biosensors & Bioelectronics
|April 26, 2003
Summary
A new, stable optical carbon dioxide (CO2) sensor was developed using a silicone matrix and HPTS dye. This autoclavable sensor offers reliable, non-invasive CO2 detection for various applications, including fermentation processes.
Area of Science:
- Chemical Sensors
- Materials Science
- Biotechnology
Background:
- Existing plastic-based CO2 sensors suffer from limitations like dye leaching and ion cross-sensitivity.
- Development of robust, non-invasive CO2 sensors is crucial for real-time monitoring in diverse applications.
Purpose of the Study:
- To fabricate and characterize a high-stability, non-invasive, autoclavable optical CO2 sensor.
- To investigate factors affecting sensor stability and performance.
- To evaluate the sensor's applicability in biological processes.
Main Methods:
- Fabrication of a silicone-based optical sensor using 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) as the fluorescent dye and cetyltrimethylammonium hydroxide (CTMAOH) as the base.
- Optimization of sensing film composition, focusing on base concentration and water content.
- Characterization of sensor performance, including stability, response/recovery times, detection limit, and cross-sensitivity.
Main Results:
- The developed sensor demonstrated high stability, overcoming limitations of existing plastic-type sensors.
- Sufficient base and a small amount of water were identified as critical for sensor stability.
- The sensor exhibited a detection limit of 0.03%, rapid response/recovery times (0.66/1.94 min), and no cross-sensitivity to varying salt concentrations and pH levels.
- The sensor maintained sensitivity after multiple autoclaving cycles and showed successful application in Escherichia coli fermentation.
Conclusions:
- A novel, highly stable, non-invasive, and autoclavable optical CO2 sensor was successfully developed.
- The silicone-based sensor offers significant advantages over existing technologies, including enhanced durability and resistance to leaching and ion interference.
- The sensor's robustness and reliability make it suitable for demanding applications, including sterile environments and biological process monitoring.