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Related Experiment Videos

Structural changes in trichocyte keratin intermediate filaments during keratinization.

R D Bruce Fraser1, Peter M Steinert, David A D Parry

  • 1Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.

Journal of Structural Biology
|April 26, 2003
PubMed
Summary

Hard alpha-keratins like hair undergo significant molecular changes during keratinization. This study models intermediate filaments (IF) in their reduced state and explores structural modifications during fiber formation.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Structural Biology

Background:

  • Hard alpha-keratins (e.g., hair, quill) feature intermediate filaments (IF) within a sulfur-rich matrix.
  • Keratinization involves cysteine oxidation, altering the molecular architecture of trichocyte keratin IF.

Purpose of the Study:

  • To develop a generic model for reduced-state IF based on cross-linking data.
  • To investigate potential structural transformations of IF during keratinization.

Main Methods:

  • Cross-linking studies to model reduced-state IF.
  • Analysis of cryo-electron microscopy and transmission electron microscopy data.

Main Results:

  • A generic model for reduced-state IF is proposed.

Related Experiment Videos

  • Two hypotheses are presented for IF structural changes during keratinization, transitioning from tubular to ring-core structures.

    • Conclusions:

    • The study provides a model for IF structure before keratinization.
    • It explores how IF molecular architecture changes during the terminal differentiation process.