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Glyceraldehyde-3-phosphate dehydrogenase expression varies with age and nutrition status
Paul E Mozdziak1, Julia J Dibner, Darell W McCoy
1Department of Poultry Science, North Carolina State University, Raleigh, North Carolina 27695, USA. pemozdzi@unity.ncsu.edu
Objective:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway, and it is a popular internal standard for northern blot analysis. We examined GAPDH expression early in life when feed is either provided or not provided to animals.
Methods:
Male broiler chickens were provided a standard starter diet plus Oasis nutritional supplement (fed group; Novus International, St. Louis, MO, USA) or no feed (starved group) for the first 3 d posthatch. Subsequently, the standard starter diet was provided to all chickens between 3 and 7 d posthatch. RNA was extracted from the pectoralis thoracicus, and GAPDH expression was evaluated with quantitative northern analysis.
Results:
GAPDH expression was significantly (P < 0.05) higher in the fed than in the starved group at 3 d posthatch, suggesting that nutritional manipulations can alter GAPDH transcription. Similarly, GAPDH mRNA levels were significantly (P < 0.05) higher at 7 d posthatch compared with all younger animals, suggesting that GAPDH is developmentally upregulated with advancing age.
Conclusion:
GAPDH expression changes with age and nutrition status in the early posthatch chick, suggesting that GAPDH is not a proper internal standard for muscle studies using quantitative northern analysis.