Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ligand density modulates eosinophil signaling and migration.

A Holub1, J Byrnes, S Anderson

  • 1Department of Pediatrics, University of Wisconsin, Madison 53706, USA.

Journal of Leukocyte Biology
|April 26, 2003
PubMed
Summary

Fibronectin, not VCAM-1, inhibits eosinophil migration and signaling in asthma. This finding clarifies how eosinophils function in airway inflammation, impacting asthma research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishing an <i>ex vivo</i> porcine skin model to investigate the effects of broad-spectrum antiseptic on viable skin microbial communities.

mSphere·2025
Same author

Structural biology at the National Synchrotron Light Source II.

Journal of synchrotron radiation·2025
Same author

Laser ablation sample preparation for atom probe tomography and transmission electron microscopy.

Ultramicroscopy·2020
Same author

International HTA Experience with Targeted Therapy Approvals for Lung Cancer.

PharmacoEconomics - open·2018
Same author

Phosphatidylethanolamine is progressively exposed in RBCs during storage.

Transfusion medicine (Oxford, England)·2017
Same author

Second harmonic generation microscopy in zebrafish.

Methods in cell biology·2016

Area of Science:

  • Immunology
  • Cell Biology
  • Respiratory Medicine

Background:

  • Eosinophils are key players in persistent airway inflammation associated with asthma.
  • The mechanisms retaining eosinophils in the airways are not fully understood.
  • Elevated fibronectin levels in asthma airways correlate with eosinophil counts.

Purpose of the Study:

  • To investigate the impact of fibronectin and vascular cell adhesion molecule 1 (VCAM-1) density on eosinophil migration and signaling.
  • To determine if fibronectin density modulates eosinophil function in the context of asthma.

Main Methods:

  • Eosinophil migration and spreading assays were performed on surfaces with varying densities of fibronectin and VCAM-1.
  • Mitogen-activated protein kinase (MAPK) signaling pathways, specifically p38 and extracellular signal-regulated kinase (ERK), were analyzed.

Related Experiment Videos

  • The effects of different fibronectin and VCAM-1 concentrations on these pathways were assessed.

    • Main Results:

    • Increasing fibronectin concentrations led to a dose-dependent inhibition of eosinophil spreading and migration.
    • VCAM-1 density did not significantly affect eosinophil migration or spreading.
    • High fibronectin concentrations inhibited p38 MAPK activation, while VCAM-1 had minimal effect.
    • Both high fibronectin and VCAM-1 concentrations slightly reduced ERK activity.

    Conclusions:

    • Fibronectin, but not VCAM-1, directly inhibits eosinophil migration and signaling pathways.
    • These findings suggest fibronectin plays a critical role in regulating eosinophil behavior within the asthmatic airway.
    • Understanding fibronectin's inhibitory role may offer new therapeutic targets for managing eosinophilic airway inflammation in asthma.