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Effect of perfluorooctyl bromide on bacterial growth
Rose Jung1, Susan L Pendland, Steven J Martin
1School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado, USA.
Background:
Perfluorooctyl bromide (PFOB) is administered directly into the lungs of critically ill patients during partial liquid ventilation. This adjunctive therapy facilitates respiratory support in lung-injured patients and potentially interacts with pathogens in patients with pneumonia. The purpose of this study was to determine the interaction of PFOB with Pseudomonas aeruginosa.
Methods:
The antimicrobial activity of PFOB against P. aeruginosa was determined using modified time-kill methods. PFOB concentrations of 25, 50, 75, 90 and 99% were studied. Electron microscopy was used to evaluate morphologic changes following PFOB exposure to the organism.
Results:
Viable counts at baseline were approximately 1 log(10) lower when P. Aeruginosa was exposed to PFOB compared to controls. Significant bacterial killing occurred over the first 2 h for the 90% (p = 0.023) and 99% (p = 0.045) PFOB concentrations versus control. Electron microscopy demonstrated that PFOB disturbs the bacterial cell wall, and produces vacuolizations within the cell.
Conclusions:
PFOB kills P. aeruginosa in a concentration-dependent manner through disruption of the cell architecture.
Insights
Perfluorooctyl bromide (PFOB) demonstrates antimicrobial activity against Pseudomonas aeruginosa. This compound kills bacteria in a concentration-dependent manner by disrupting the bacterial cell wall and internal structures.
Area of Science:
- Microbiology
- Pulmonology
- Biomedical Engineering
Background:
- Perfluorooctyl bromide (PFOB) is used in partial liquid ventilation for critically ill patients.
- PFOB may interact with bacterial pathogens like Pseudomonas aeruginosa in pneumonia patients.
Purpose of the Study:
- To determine the interaction between PFOB and Pseudomonas aeruginosa.
- To evaluate the antimicrobial activity of PFOB against P. aeruginosa.
Main Methods:
- Modified time-kill assays were employed to assess antimicrobial activity.
- PFOB concentrations ranging from 25% to 99% were tested.
- Electron microscopy visualized morphological changes in P. aeruginosa post-exposure.
Main Results:
- PFOB exposure reduced viable P. aeruginosa counts compared to controls.
- Significant bacterial killing was observed at 90% and 99% PFOB concentrations within 2 hours.
- Electron microscopy revealed cell wall disruption and intracellular vacuolization.
Conclusions:
- PFOB exhibits concentration-dependent killing of P. aeruginosa.
- The mechanism involves disruption of bacterial cell architecture.