Activity of protein kinase A and gustatory responsiveness in the honey bee ( Apis mellifera L.)

The cAMP-dependent protein kinase A is involved in the induction of long-term memory and habituation in the bee. Gustatory responsiveness correlates strongly with associative and non-associative learning in bees. We tested whether protein kinase A activity in the antennal lobes correlates with gustatory responsiveness. Thirty minutes after feeding, bees with high gustatory responsiveness had a significantly higher protein kinase A activity than bees with low responsiveness. Ninety minutues after feeding, when gustatory responsiveness had increased in initially unresponsive bees, no changes in protein kinase A activity were found. We also tested time-dependent effects of protein kinase A activator and protein kinase A inhibitor on gustatory responsiveness. Injection of the protein kinase A activator adenosine 3'5'-cyclic monophosphate 8-bromo-sodium salt or of the protein kinase A inhibitor KT 5720 did not affect gustatory responsiveness within the first 4 h after treatment. Feeding of adenosine 3'5'-cyclic monophosphate 8-bromo-sodium salt over 4 days increased gustatory responsiveness in newly emerged bees and adult foragers. These results enable us to distinguish between two different forms of gustatory responsiveness: basal and transient gustatory responsiveness. Basal gustatory responsiveness correlates with protein kinase A activity and can only be modulated in the range of several days. Transient gustatory responsiveness appears to be independent of protein kinase A activity and can be modulated in the range of minutes to hours.