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Optimization of phytase production by solid substrate fermentation.

B Bogar1, G Szakacs, J C Linden

  • 1Department of Agricultural Chemical Technology, Technical University of Budapest, Gellert ter 4, 1111, Budapest, Hungary.

Journal of Industrial Microbiology & Biotechnology
|April 26, 2003
PubMed
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This study optimized phytase production using Aspergillus ficuum in solid substrate fermentation (SSF). Optimized wheat bran medium boosted phytase yield, achieving consistent results upon scaling up for feed applications.

Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Agricultural Microbiology

Background:

  • Filamentous fungi are key producers of industrial enzymes.
  • Solid substrate fermentation (SSF) offers advantages for enzyme production using agricultural byproducts.
  • Phytase is crucial for improving phosphorus availability in animal feed.

Purpose of the Study:

  • To evaluate phytase production by selected filamentous fungi on various feed ingredients.
  • To optimize conditions for enhanced phytase yield using Aspergillus ficuum.
  • To assess enzyme production scalability in different SSF systems.

Main Methods:

  • Screening of Aspergillus ficuum, Mucor racemosus, and Rhizopus oligosporus for phytase production.
  • Solid substrate fermentation (SSF) using canola meal, cracked corn, soybean meal, and wheat bran.

Related Experiment Videos

  • Optimization of wheat bran medium with starch and ammonium sulfate using Plackett-Burman and central composite designs.
  • Enzyme activity assays for phytase, phosphatase, alpha-amylase, and xylanase.
  • Scalability assessment from Erlenmeyer flasks to tray SSF.

    • Main Results:

    • Aspergillus ficuum NRRL 3135 exhibited the highest phytase yield (15 IU/g dry matter) on wheat bran.
    • Optimized supplementation increased phytase production to 25 IU/g dry matter.
    • Scalable SSF in trays reproducibly yielded 20 IU phytase activity/g dry matter.

    Conclusions:

    • Aspergillus ficuum is a promising candidate for high-yield phytase production via SSF.
    • Optimization of substrate and fermentation conditions significantly enhances enzyme output.
    • Tray-based SSF provides a viable method for scaling up phytase production for feed applications.