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Updated: Sep 26, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
Published on: June 6, 2014
[Automated analysis of reticulocytes]
Abstract:
An evaluation of the performance of the GEN-S analyzer in examining the parameters of reticulocytes is described. The automated and manual methods of counting the relative quantity of reticulocytes were compared. It was established that the precision of the analyzer significantly surpasses the manual method results. The variation coefficient for the parameters of examination of reticulocytes is lower at a higher content of reticulocytes and the accuracy of the method is better if the content of reticulocytes in blood is higher. The parameters, characterizing the fraction of immature reticulocytes, are least accurate. The study demonstrated that the automated method of analyzing the reticulocytes by using the GEN-S analyzer can be used to ensure a more accurate monitoring of changes of the function of erythropoesis at different anemic conditions and to assess the conducted therapy.

