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[Analysis of sperm chromosomes in infertile males with teratozoospermia]
Monika A Szczygieł1, Hirokazu Kusakabe, Ewa Wiland
1Zespołu Biologii Rozrodu, Zakładu Genetyki Człowieka PAN.
Objectives:
The aim of the study was to examine sperm chromosomes from infertile males with teratozoospermia.
Design:
Basic research study
Materials And Methods:
Spermatozoa were obtained from infertile males with teratozoospermia. Intracytoplasmic sperm injection was used to inject human spermatozoa into mouse oocytes in order to examine sperm chromosomes.
Results:
Chromosomes of spermatozoa obtained from 6 infertile teratozoospermic and 1 fertile normozoospermic males were investigated. The mean frequency of structural chromosome aberrations in amorphous, motile spermatozoa was similar to that of morphologically normal sperm (9 vs. 7.7%). The structural aberrations found in amorphous, immotile spermatozoa reached 93% and revealed severity was significant.
Conclusions:
Teratozoospermia per se did not increase the frequency of structural chromosome aberrations in sperm. Severe chromosomal damage was revealed when immotile spermatozoa were injected into oocytes by ICSI.