Related Experiment Video
Updated: Aug 14, 2026
Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Published on: March 28, 2014
Effect of intermittent radiant warming on proliferation of human dermal endothelial cells in vitro
M A Hughes1, C Tang, G W Cherry
1Oxford Wound Healing Institute, The Churchill Hospital, Oxford, UK. oxfordwound@aol.com
Objective:
This study investigated the effect of Warm-Up therapy on the proliferation of human microvascular dermal endothelial cells.
Method:
Endothelial cells from an adult subject were seeded in six-well plates and placed in an incubator at 32.5 degrees C. The following day Warm-Up dressings were placed over the plates, with or without warming cards. Cards set at 38 degrees C or 42 degrees C raised the temperature in the medium to maxima of 34.5 degrees C and 37.5 degrees C respectively. Units were switched on daily for three one-hour periods. Cell numbers were counted by haemocytometer.
Results:
Maximum stimulation of endothelial cell proliferation occurred under the 38 degrees C card, with cells numbering 135-158% of the controls (p < 0.05). the 42 degrees C card also stimulated cells (110-155%) but this did not reach statistical significance.
Conclusion:
The accelerated proliferation of microvascular dermal endothelial cells achieved by intermittent radiant warming may have contributed to the increase in granulation tissue reported previously in our clinic.
