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[Preliminary study on hepatitis B virus quasispecies in a patient with chronic hepatitis B]
Lin Lan1, Yu-ming Wang, Yan-ping Huang
1Institute of Infectious Diseases of the People's Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Objective:
To investigate whether the hepatitis B virus (HBV) has quasispecies character by studying nucleotide sequence polymorphism and mutation features of HBV PreC/C gene region, and preliminaryly explore the heterogeneity of HBV quasispecies.
Methods:
The serum sample was obtained from a patient with chronic hepatitis B, and the whole HBV PreC/C gene region was amplified by PCR and cloned. Thirty-four clones that contained HBV PreC/C gene fragments were sequenced.
Results:
There were 28 kinds of different nucleotide sequences in 34 clones, and the nucleotide sequences diversity ranged from 0.2% to 2.1%. The mutation points were almost distributed in the whole region, but there wasn't mutation at PreC region nt.1 896 point in all sequences.
Conclusion:
Hepatitis B virus has complex quasispecies character in the patients with chronic hepatitis B.