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Related Experiment Videos

SHED: stem cells from human exfoliated deciduous teeth.

Masako Miura1, Stan Gronthos, Mingrui Zhao

  • 1Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 30, 2003
PubMed
Summary

Human exfoliated deciduous teeth contain multipotent stem cells (SHED). These stem cells can differentiate into various cell types and show potential for regenerative medicine and tissue engineering applications.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Dental Research

Background:

  • Isolating high-quality human postnatal stem cells from accessible sources is crucial for research.
  • Exfoliated deciduous teeth represent a readily available biological resource.

Purpose of the Study:

  • To identify and characterize stem cells within exfoliated human deciduous teeth.
  • To evaluate the potential of these stem cells for therapeutic applications.

Main Methods:

  • Isolation and culture of stem cells from human exfoliated deciduous teeth (SHED).
  • Assessment of stem cell proliferation, clonogenicity, and differentiation potential in vitro.
  • In vivo transplantation studies to evaluate bone formation, dentin generation, and neural marker expression in mouse models.

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Main Results:

  • SHED were identified as a population of highly proliferative and clonogenic cells.
  • SHED demonstrated differentiation capacity into neural cells, adipocytes, and odontoblasts.
  • In vivo studies showed SHED could induce bone and dentin formation and survive in the mouse brain expressing neural markers.

Conclusions:

  • Exfoliated deciduous teeth harbor a unique population of multipotent stem cells (SHED).
  • SHED possess significant potential for stem cell therapies, including autologous transplantation and tissue engineering.
  • SHED offer an accessible and promising source for regenerative medicine.