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Biochemical changes in apparently normal sheep from flocks affected by polioencephalomalacia
1Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, C/Miguel Servet 177, 50013 Zaragoza, Spain. jjramos@posta.unizar.es
Veterinary Research Communications
|April 30, 2003
Summary
Diagnosing Polioencephalomalacia (PEM) in sheep may be aided by measuring erythrocyte transketolase activity and fecal thiaminase levels. These indicators can help identify high-risk periods for PEM development, particularly in replacement ewes during spring.
Area of Science:
- Veterinary Medicine
- Biochemistry
- Animal Science
Background:
- Polioencephalomalacia (PEM) is a significant neurological disease in sheep.
- Understanding biochemical markers and risk factors is crucial for preventing PEM outbreaks.
Purpose of the Study:
- To evaluate erythrocyte transketolase activity and fecal thiaminase as potential diagnostic markers for PEM risk in sheep.
- To investigate the role of diet and microbial thiaminase production in PEM development.
Main Methods:
- Blood samples were analyzed for lactate, pyruvate, and erythrocyte transketolase activity.
- Fecal samples were collected for thiaminase estimation.
- Feed and pasture grass samples were analyzed for thiaminase content.
Main Results:
- Decreased erythrocyte transketolase activity correlated with an increased thiamin pyrophosphate effect.
- Fecal thiaminase levels were estimated in healthy, control, and PEM-affected sheep.
- Dietary changes were implicated in microbial thiaminase production, with higher prevalence in spring.
Conclusions:
- Erythrocyte transketolase activity and fecal thiaminase estimation may serve as valuable diagnostic tools for identifying periods of increased PEM risk.
- Dietary shifts are likely triggers for thiaminase production, contributing to PEM in sheep, especially during spring.