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Expression of AP-1 (c-fos/c-jun) in developing mouse corneal epithelium
Yuka Okada1, Shizuya Saika, Kumi Shirai
1Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, 641-0012 Wakayama, Japan. yokada@wakayama-med.ac.jp
Background:
Activator protein-1 (AP-1) is a ubiquitous transcription factor which is believed to modulate cell behaviors such as proliferation and differentiation during wound healing and embryonic tissue morphogenesis. AP-1 consists of Fos family and Jun family proteins.
Methods:
We examined expression pattern of c-fos mRNA and c-Fos protein in developing mouse cornea. Expression of c-jun mRNA and c-Jun protein were also examined for comparison.
Results:
While no c-fos mRNAs were detected on embryonic day (E) 12.5, mRNA for c-fos was detected from E14.5 until postnatal day (P)14. The mRNAs for c-jun were also detected, although the temporal expression patterns differed. c-Fos-immunoreactive nuclei were present from E14.5 through P10 and c-Jun-immunoreactive nuclei were detected from E14.5 through P3.
Conclusion:
These findings indicate that AP-1 ( c-fos/c-jun) transcription factor may play a role in the development and maturation of the corneal epithelium in mice.