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Published on: February 27, 2021
Phenylboronate-chitosan resins for adsorption of beta-amylase from soybean extracts
Eduardo J Arruda1, Cesar C Santana
1Pharmacy and Biochemistry, Dom Bosco Catholic University, UCDB, C.P. 100, CEP 79117-900, Campo Grande, MS, Brazil.
Abstract:
Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a beta-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of beta-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.

