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[Estimation of DNA methylation level in nonendometrial uterus malignancies]
Andrzej Popiela1, Marian Stanisław Gabryś, Jerzy Rabczyński
1II Katedry i Kliniki Ginekologii AM we Wrocławiu.
Objectives:
Rebuilding of genome structure leads to many pathological states including neoplastic malignancies. Rebuilding often occurs as a process caused by disturbances in gene silencing mechanism. DNA methylation pattern is one of the most important mechanisms connected to gene's silencing. Estimation of DNA methylation level in nonendometrial uterine neoplastic tissues compared to normal endometrial samples was the aim of this study.
Design:
It was to be shown, that changes in methylation rate in promotory regions could lead to carcinogenesis in particular cell. Authors describe an analysis of DNA methylation level in nonendometrial neoplastic uterine tissues compared to DNA methylation level in normal endometrium.
Materials And Methods:
Tissue samples from 9 women with tumor mixtus mesodermalis were collected. 12 samples were normal endometrium-control group. DNA was isolated from tissues and than we performed an estimation of DNA methylation levels. Than we performed a statistical analysis of results.
Results:
The median DNA methylation level was significantly higher in neoplastic tissues than in normal endometrium.
Conclusions:
Authors conclude, that DNA methylation level is higher in neoplastic tissues, but does not correlate with clinical stage of the disease.