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Microvascular Embolism Mouse Model for In Vivo Two-photon Microscopy Using Fluorescent Polystyrene Microspheres
Published on: November 21, 2025
Brain microembolism
Ahmed Aly1, Viken L Babikian, Glenn Barest
1Department of Neurology, Department of Radiology, Boston University School of Medicine, Harvard Medical School, MA, USA.
Purpose:
To alert clinicians about the occurrence of a subtype of brain infarction, its suspected etiology, and its detection by specific neuroimaging techniques.
Methods:
The article presents 5 nonconsecutive patients admitted to the stroke services of 2 tertiary care hospitals, who presented with acute or subacute symptoms suspicious, but at times atypical, of brain ischemia.
Findings:
Each patient had evidence of 3 to > 20 small areas of recent brain infarction detected by diffusion-weighted imaging (DWI). When available, brain computerized tomography images were not helpful for the diagnosis of these recent infarcts. Most lesions were present on magnetic resonance imaging fluid-attenuated inversion recovery sequences, but the diffusion-weighted images allowed the determination of their acuity. Further evaluation revealed a potential source of embolism in each patient. Brain microembolism was suspected in all cases.
Conclusion:
Small and multiple areas of acute or subacute brain infarction occasionally present with clinical features atypical for brain embolism. They can be detected by magnetic resonance DWI studies.
Insights
Clinicians should be aware of atypical brain infarction presentations. Magnetic resonance diffusion-weighted imaging (DWI) effectively detects these small, multiple acute infarcts, often linked to microembolism.
Area of Science:
- Neurology
- Neuroimaging
- Stroke Medicine
Background:
- Brain infarction subtypes require precise diagnostic approaches.
- Early detection of acute ischemic events is crucial for patient outcomes.
Observation:
- Five patients presented with atypical symptoms suggestive of brain ischemia.
- Computed tomography (CT) scans were insufficient for diagnosing these acute infarcts.
Findings:
- Diffusion-weighted imaging (DWI) identified 3 to over 20 small, recent brain infarcts per patient.
- Magnetic resonance imaging (MRI) fluid-attenuated inversion recovery (FLAIR) sequences showed most lesions.
- DWI was essential for determining the acuity of the infarcts, with suspected microembolism as the etiology.
Implications:
- This subtype of brain infarction may present with subtle or atypical clinical signs.
- Advanced MRI techniques, particularly DWI, are vital for accurate diagnosis and characterization.
- Recognizing these findings aids in identifying potential embolic sources and guiding treatment.
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