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Updated: Aug 14, 2026

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Published on: September 1, 2009
[The frequency of heteroploidy in spermatozoa of patients with infertility]
I D Fedorova1, Iu A Loginova, O A Leont'eva
1Ott Institute of Obstetrics and Gynecology, Russian Academy of Medical Sciences, St. Petersburg, 199034 Russia.
Abstract:
The incidence of heteroploid spermatozoa was studied by mono- and dual-colored fluorescence in situ hybridization in semen samples from donors and patients with normal and impaired spermatogenesis. The frequency of heteroploid sperm in the ejaculate was linearly and inversely correlated with sperm parameters (sperm concentration in the ejaculate and proportion of motile and morphologically normal spermatozoa). The level of heterploidy was the most significant in the semen samples from patients with oligoasthenoteratospermia and oligoasthenospermia.
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